Hiemstra H S, van Veelen P A, Geluk A, Schloot N C, de Vries R R, Ottenhoff T H, Roep B O, Drijfhout J W
Department of Immunohematology and Blood Bank, Leiden University Medical Center, Leiden, Albinusdreef 2, P.O. box 9600, 2300 RC, Leiden, Netherlands.
Vaccine. 1999 Sep;18(3-4):204-8. doi: 10.1016/s0264-410x(99)00328-x.
Mimicry epitopes that are recognized by T-cells can be identified through screening of synthetic peptide libraries. We have shown that these mimicry epitopes share sequence similarity with the corresponding natural epitopes and that mimicry sequences can be used for the definition of protein derived T-cell epitopes from databases. This can be done by either homology searching or pattern searching. Here we discuss the advantages and disadvantages of homology searching as an alternative for the generally applicable recognition pattern approach. We show that only for part of the library derived mimicry epitopes, the degree of similarity to the natural epitope may be high enough for successful homology searching in small databases.
可通过筛选合成肽文库来鉴定被T细胞识别的模拟表位。我们已经表明，这些模拟表位与相应的天然表位具有序列相似性，并且模拟序列可用于从数据库中定义蛋白质衍生的T细胞表位。这可以通过同源性搜索或模式搜索来完成。在这里，我们讨论同源性搜索作为普遍适用的识别模式方法的替代方法的优缺点。我们表明，仅对于部分文库衍生的模拟表位，与天然表位的相似程度可能足够高，以便在小型数据库中成功进行同源性搜索。
