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Improved preparation and structural elucidation of the tryptophan cleavage reagent 2-(2'-nitro-phenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole).

作者信息

Zeitler H J, Eulitz M

出版信息

J Clin Chem Clin Biochem. 1978 Dec;16(12):669-74. doi: 10.1515/cclm.1978.16.12.669.

DOI:10.1515/cclm.1978.16.12.669
PMID:105078
Abstract

Methods are described for a high yield preparation (overall yield greater than 90%) of analytically pure 2-(2'-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole). The chromatographic and spectroscopic data are given. The yield of tryptophan cleavage is considerably dependent on the degree of purity of the BNPS-skatole.

摘要

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1
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引用本文的文献

1
Chemical cleavage of bovine beta-lactoglobulin by BNPS-skatole for preparative purposes: comparative study of hydrolytic procedures and peptide characterization.
J Protein Chem. 1999 Jan;18(1):1-12. doi: 10.1023/a:1020635130077.