Kost T A, Condreay J P
Molecular Sciences Department Glaxo Wellcome 5 Moore Drive, Research Triangle Park, NC 27709, USA.
Curr Opin Biotechnol. 1999 Oct;10(5):428-33. doi: 10.1016/s0958-1669(99)00005-1.
Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein production, and additional applications of baculovirus display technology. The application of modified baculovirus vectors for gene expression in mammalian cells continues to expand.
杆状病毒表达载体被广泛用于在培养的昆虫细胞中表达异源蛋白。最近的进展包括用于生产多亚基蛋白复合物的系统的进一步发展、共表达蛋白质修饰酶以提高异源蛋白产量以及杆状病毒展示技术的更多应用。修饰的杆状病毒载体在哺乳动物细胞中用于基因表达的应用也在不断扩大。
