Kovall R A, Matthews B W
Institute of Molecular Biology, Howard Hughes Medical Institute, Department of Physics, 1229 University of Oregon, Eugene, OR 97403-1229, USA.
Curr Opin Chem Biol. 1999 Oct;3(5):578-83. doi: 10.1016/s1367-5931(99)00012-5.
Type II restriction endonucleases are a paradigm for site-specific cleavage of DNA. Recent structural analyses, in particular in the presence of various divalent metals, have shed new insight into the mechanisms of catalysis. In addition, during this past year the crystal structure determinations of MutH, lambda-exonuclease and FokI have revealed that these proteins are also members of the same family.
II型限制性内切核酸酶是DNA位点特异性切割的范例。最近的结构分析，特别是在各种二价金属存在的情况下，为催化机制提供了新的见解。此外，在过去的一年里，MutH、λ-外切核酸酶和FokI的晶体结构测定表明，这些蛋白质也是同一家族的成员。
