Gilliespie B E, Jayarao B M, Dowlen H H, Oliver S P
Department of Animal Science, University of Tennessee, Knoxville 37996, USA.
J Dairy Sci. 1999 Sep;82(9):2049-53. doi: 10.3168/jds.S0022-0302(99)75443-3.
Jersey cows (n = 172) were genotyped for the bovine lymphocyte antigen (BoLA)-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism analysis. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, BstyI, and HaeIII was conducted on the DNA from Jersey cattle. Twenty-four BoLA-DRB3.2 alleles were identified with frequencies ranging from 0.3 to 22.9%. Thirteen allele types were similar to those reported previously; and 11 were new allele types that have not been reported previously. Allele types reported previously include: BoLA-DRB3.2*2, *8, *10, *15, *17, *20, *21, *22, *23, *25, *28, *36, and *37. Their frequencies were 0.3, 11.3, 22.9, 13.6, 5.5, 3.7, 10.7, 3.5, 0.9, 0.3, 4.7, 9.3, and 0.9%, respectively. Of the new allele types detected, ibe occurred at the highest frequency (6.1%) in Jersey cows from this herd. The six most frequently isolated alleles (BoLA-DRB3.28, *10, *15, *21, *36, and *ibe) accounted for 73.9% of the alleles in the population of this herd. Results of this study demonstrate that the BoLA-DRB3.2 locus is highly polymorphic in Jersey cattle.
通过聚合酶链反应和限制性片段长度多态性分析，对172头泽西奶牛的牛淋巴细胞抗原（BoLA）-DRB3.2等位基因进行基因分型。从全血等分试样中分离牛DNA。对泽西牛的DNA进行两步聚合酶链反应，随后用限制性内切酶RsaI、BstyI和HaeIII进行消化。鉴定出24个BoLA-DRB3.2等位基因，频率范围为0.3%至22.9%。13种等位基因类型与先前报道的相似；11种是以前未报道过的新等位基因类型。先前报道的等位基因类型包括：BoLA-DRB3.2*2、*8、*10、*15、*17、*20、*21、*22、*23、*25、*28、36和37。它们的频率分别为0.3%、11.3%、22.9%、13.6%、5.5%、3.7%、10.7%、3.5%、0.9%、0.3%、4.7%、9.3%和0.9%。在所检测到的新等位基因类型中，ibe在该牛群的泽西奶牛中出现频率最高（6.1%）。六种最常分离的等位基因（BoLA-DRB3.28、*10、*15、*21、36和ibe）占该牛群等位基因的73.9%。本研究结果表明，BoLA-DRB3.2基因座在泽西牛中具有高度多态性。
