Caffesse R G, Nasjleti C E, Castelli W A
J Periodontol. 1979 Jan;50(1):1-6. doi: 10.1902/jop.1979.50.1.1.
The influence of the sulcular environment on the keratinization of the outer surface gingival epithelium was tested in three young adult Rhesus monkeys. A total of 40 mucoperiosteal flaps were raised, and inverted so as to bring the outer surface epithelium in contact with the tooth, and were sutured. The experimental time intervals varied from 1 hour to 60 days. The monkeys received H3 thymidine 1 hour prior to sacrifice. The material was prepared for histologic and radioautographic evaluation. Results indicated that the outer surface epithelium changes its morphology to a nonkeratinized epithelium devoid of deep rete pegs when in close contact with the tooth, resulting in the anatomical characteristics normally seen in sulcular epithelium. It is concluded that the sulcular environment has the capability of controlling the keratinizing potential of the outer surface gingival epithelium. It is further suggested that the constant irritation of bacterial plaque and its products may be responsible for the premature desquamation of the sulcular epithelium which in turn might not allow its full differentiation.
在三只成年恒河猴身上测试了龈沟环境对牙龈外表面上皮角化的影响。总共掀起并翻转了40个黏骨膜瓣，以使外表面上皮与牙齿接触，然后进行缝合。实验时间间隔从1小时到60天不等。在处死前1小时给猴子注射H3胸腺嘧啶核苷。对材料进行组织学和放射自显影评估。结果表明，当外表面上皮与牙齿紧密接触时，其形态会转变为无深 rete 嵴的非角化上皮，从而形成龈沟上皮通常所见的解剖特征。结论是龈沟环境有能力控制牙龈外表面上皮的角化潜能。进一步表明，菌斑及其产物的持续刺激可能是龈沟上皮过早脱落的原因，而这反过来可能不允许其充分分化。
