拟南芥细胞悬浮培养中的抗坏血酸生物合成
Ascorbate biosynthesis in Arabidopsis cell suspension culture.
作者信息
Davey M W, Gilot C, Persiau G, Ostergaard J, Han Y, Bauw G C, Van Montagu M C
机构信息
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
出版信息
Plant Physiol. 1999 Oct;121(2):535-43. doi: 10.1104/pp.121.2.535.
Abstract
The biosynthesis of L-ascorbic acid (L-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of L-AA. Our results support the recently published biosynthetic pathway of L-AA from L-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365-369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading to L-AA. The possible physiological significance of these alternate routes is discussed.
摘要
通过量化一系列潜在生物合成前体、类似物和抑制剂对拟南芥（Arabidopsis (L.) Heynh.）细胞悬浮培养物中还原型和氧化型L-抗坏血酸（L-AA）细胞内水平的影响，研究了L-抗坏血酸（L-AA）的生物合成。我们的结果支持最近发表的从L-半乳糖合成L-AA的生物合成途径（G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365 - 369），但表明拟南芥细胞悬浮培养物同时还包含另外两条合成L-AA的途径。文中讨论了这些替代途径可能的生理意义。
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Direct measurement of ascorbic acid biosynthesis in Arabidopsis cell suspension culture using capillary electrophoresis.利用毛细管电泳直接测量拟南芥细胞悬浮培养物中抗坏血酸的生物合成。
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Biosynthesis of ascorbic acid in kidney bean. L-galactono-gamma-lactone dehydrogenase is an intrinsic protein located at the mitochondrial inner membrane.菜豆中抗坏血酸的生物合成。L-半乳糖酸-γ-内酯脱氢酶是一种位于线粒体内膜的内在蛋白。
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