Davey M W, Gilot C, Persiau G, Ostergaard J, Han Y, Bauw G C, Van Montagu M C
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Plant Physiol. 1999 Oct;121(2):535-43. doi: 10.1104/pp.121.2.535.
The biosynthesis of L-ascorbic acid (L-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of L-AA. Our results support the recently published biosynthetic pathway of L-AA from L-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365-369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading to L-AA. The possible physiological significance of these alternate routes is discussed.
通过量化一系列潜在生物合成前体、类似物和抑制剂对拟南芥(Arabidopsis (L.) Heynh.)细胞悬浮培养物中还原型和氧化型L-抗坏血酸(L-AA)细胞内水平的影响,研究了L-抗坏血酸(L-AA)的生物合成。我们的结果支持最近发表的从L-半乳糖合成L-AA的生物合成途径(G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365 - 369),但表明拟南芥细胞悬浮培养物同时还包含另外两条合成L-AA的途径。文中讨论了这些替代途径可能的生理意义。