衍生化纤维素和直链淀粉手性固定相在高效液相色谱法分离药物化合物对映体中的应用与比较
Application and comparison of derivatized cellulose and amylose chiral stationary phases for the separation of enantiomers of pharmaceutical compounds by high-performance liquid chromatography.
作者信息
Wang T, Chen Y W
机构信息
Analytical Research Department, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
出版信息
J Chromatogr A. 1999 Sep 10;855(2):411-21. doi: 10.1016/s0021-9673(99)00733-5.
The direct high-performance liquid chromatographic separation of three pairs of structurally related enantiomers on derivatized cellulose and amylose chiral stationary phases (Chiralcel OD, Chiralpak AD and Chiralpak AS) was studied using hexane as the mobile phase with 2-propanol or ethanol as modifiers. The separation, retention and elution order of the enantiomers on the different columns using different alcohol modifiers were compared. The effect of structural variation of the solutes on their k' was noted. A reversal of elution order of one enantiomeric pair upon changing the mobile-phase modifier was observed. Chiralcel OD and Chiralpak AD columns provided different elution orders of the enantiomers, including a fourth pair of enantiomers that were not structurally related to the other three pairs.
研究了在衍生化纤维素和直链淀粉手性固定相（Chiralcel OD、Chiralpak AD和Chiralpak AS）上，以正己烷为流动相、2-丙醇或乙醇为改性剂，对三对结构相关对映体进行直接高效液相色谱分离。比较了使用不同醇类改性剂时，对映体在不同色谱柱上的分离、保留和洗脱顺序。记录了溶质结构变化对其容量因子（k'）的影响。观察到改变流动相改性剂时，一对对映体的洗脱顺序发生了反转。Chiralcel OD柱和Chiralpak AD柱提供了不同的对映体洗脱顺序，包括第四对对映体，其结构与其他三对不相关。
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