Yamanaka H, He X, Matsumoto K, Shiosaka S, Yoshida S
Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan.
Brain Res Mol Brain Res. 1999 Aug 25;71(2):217-24. doi: 10.1016/s0169-328x(99)00187-4.
No proteases have been identified to be oligodendrocyte-specific though there are phenomena in which proteases may be committed. We have cloned a cDNA for mouse homologue of protease M/neurosin, serine protease, and localized its mRNA in the mouse brain. The cDNA and the deduced amino acid sequences were 66% identical to those of human protease M/neurosin. In situ hybridization histochemistry revealed that the mRNA was localized in the mature oligodendrocytes which expressed proteolipid protein mRNA. Developmentally, protease M/neurosin mRNA was expressed by oligodendrocytes 2 to 7 days after the maturation. Thus, protease M/neurosin may be important to the processes occurring after the maturation of oligodendrocytes such as myelination or turnover of the proteins in the myelin.
尽管存在蛋白酶可能参与其中的现象，但尚未鉴定出对少突胶质细胞具有特异性的蛋白酶。我们已经克隆了丝氨酸蛋白酶M/神经氨酸酶的小鼠同源物的cDNA，并将其mRNA定位在小鼠脑中。该cDNA和推导的氨基酸序列与人蛋白酶M/神经氨酸酶的序列有66%的同一性。原位杂交组织化学显示，该mRNA定位于表达蛋白脂蛋白mRNA的成熟少突胶质细胞中。在发育过程中，少突胶质细胞在成熟后2至7天表达蛋白酶M/神经氨酸酶mRNA。因此，蛋白酶M/神经氨酸酶可能对少突胶质细胞成熟后发生的过程很重要，如髓鞘形成或髓鞘中蛋白质的更新。
