Reid H I, Riggio M P
Infection Research Group, University of Glasgow Dental School, UK.
DNA Seq. 1998;9(5-6):359-64. doi: 10.3109/10425179809008477.
Bacteroides forsythus is a Gram negative anaerobe associated with human periodontal disease. Heat shock proteins are immunodominant antigens in bacteria that can elicit strong and protective immune responses. The gene specifying the GroEL protein (hsp60, heat shock protein 60) of B. forsythus was isolated by PCR amplification using consensus primers based upon published nucleotide sequences of the groEL genes of several bacterial species. Translation of the gene sequence predicts a protein of 544 amino acids in length with a molecular mass of 58 kDa. B. forsythus GroEL demonstrates identities of 50 to 81% with the predicted amino acid sequences of GroEL proteins of several bacterial species and the human mitochondrial P1 protein.
福赛斯坦纳菌是一种与人类牙周疾病相关的革兰氏阴性厌氧菌。热休克蛋白是细菌中的免疫显性抗原，可引发强烈的保护性免疫反应。基于几种细菌物种groEL基因已发表的核苷酸序列，使用共有引物通过PCR扩增分离出福赛斯坦纳菌中指定GroEL蛋白（hsp60，热休克蛋白60）的基因。该基因序列的翻译预测出一种长度为544个氨基酸、分子量为58 kDa的蛋白质。福赛斯坦纳菌GroEL与几种细菌物种的GroEL蛋白以及人类线粒体P1蛋白的预测氨基酸序列具有50%至81%的同一性。
