早产和足月婴儿的钠钾三磷酸腺苷酶活性及其在成熟过程中钠稳态中的可能作用。

Sodium potassium adenosine triphosphatase activity in preterm and term infants and its possible role in sodium homeostasis during maturation.

作者信息

Bistritzer T, Berkovitch M, Rappoport M J, Evans S, Arieli S, Goldberg M, Tavori I, Aladjem M

机构信息

Department of Paediatrics, Assaf Hroheh Medical Center, Zerifin 70300, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

出版信息

Arch Dis Child Fetal Neonatal Ed. 1999 Nov;81(3):F184-7. doi: 10.1136/fn.81.3.f184.

DOI:10.1136/fn.81.3.f184

PMID:10525020

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1721006/

Abstract

AIM

To investigate sodium (NA(+)) potassium (K(+)) adenosine triphosphatase (ATPase) activity in newborn infants at different gestational ages, to elucidate the mechanism underlying poor renal sodium conservation in preterm infants.

METHODS

Fifty three healthy newborn infants, gestational age 30-42 weeks, were studied. Umbilical cord red blood cell Na(+) K(+)ATPase activity, plasma renin activity, and plasma aldosterone activities were measured in all of them. Red blood cell Na(+) K(+)ATPase activity was re-examined in eight preterm infants, one and two weeks after birth. Total and ouabain sensitive ATPase activity was measured spectrophotometrically using a method that couples ATP hydrolysis with NADH oxidation.

RESULTS

Red blood cell Na(+) K(+)ATPase activity was significantly lower (p<0.01) in preterm babies with a gestational age below 35 weeks, compared with those with aged 35 weeks and above: 2.3 (0.8) and 6.7 (1.3) nmol NADH/minute/mg protein, respectively. There was no correlation between gestational age, Na(+) K(+)ATPase, plasma renin activity and aldosterone values either in the preterm or term babies. Two weeks after birth, irrespective of gestational age, the enzyme activity of the preterm babies increased to values similar to those observed in the term neonates at birth.

CONCLUSION

The differences in sodium homeostasis between term and preterm babies are modulated via changes in Na(+) K(+)ATPase activity.

摘要

目的

研究不同胎龄新生儿的钠（Na⁺）钾（K⁺）腺苷三磷酸酶（ATP酶）活性，以阐明早产儿肾钠保存能力差的潜在机制。

方法

对53例胎龄为30 - 42周的健康新生儿进行研究。检测所有新生儿的脐血红细胞Na⁺K⁺ATP酶活性、血浆肾素活性和血浆醛固酮活性。对8例早产儿在出生后1周和2周复查红细胞Na⁺K⁺ATP酶活性。采用将ATP水解与NADH氧化偶联的方法，通过分光光度法测定总ATP酶活性和哇巴因敏感的ATP酶活性。

结果

胎龄低于35周的早产儿红细胞Na⁺K⁺ATP酶活性显著低于（p<0.01）胎龄35周及以上的早产儿：分别为2.3（0.8）和6.7（1.3）nmol NADH/分钟/毫克蛋白。无论是早产儿还是足月儿，胎龄、Na⁺K⁺ATP酶、血浆肾素活性和醛固酮值之间均无相关性。出生后2周，无论胎龄如何，早产儿的酶活性均增加至与出生时足月儿相似的值。