Beltz R E, Herrmann E C, Nordbø H
Department of Biochemistry, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
Caries Res. 1999 Nov-Dec;33(6):468-72. doi: 10.1159/000016553.
The aim of this study was to investigate the individual capabilities of the proteolytic enzyme preparation Pronase, the enzyme collagenase and sodium hypochlorite to disintegrate and solubilize carious dentin. Samples of carious dentin, and samples of sound dentin for comparison, were extracted 4 times in succession for 24 h with buffered solutions of Pronase. Separate carious dentin samples were extracted in the same manner with buffered solutions of collagenase or with aqueous sodium hypochlorite. The extracts, the solid residues left over after the extractions and untreated samples of carious and sound dentin were digested with sulfuric acid-H(2)O(2) and then analyzed for nitrogen content by a special adaptation of the Berthelot color reaction. Although Pronase did not attack sound dentin, it solubilized more than 90% of the nitrogen present in carious dentin. Collagenase solubilized approximately 66% of the nitrogen, whereas sodium hypochlorite released only 12-20% of the nitrogen of carious dentin. In clinical dentistry, chemical disintegration of carious dentin may reduce the need for mechanical removal of sound tooth structure.
本研究的目的是调查蛋白水解酶制剂链霉蛋白酶、胶原酶和次氯酸钠分解和溶解龋坏牙本质的个体能力。龋坏牙本质样本以及用于比较的健康牙本质样本，用链霉蛋白酶缓冲溶液连续提取4次，每次24小时。分别用胶原酶缓冲溶液或次氯酸钠水溶液以相同方式提取龋坏牙本质样本。提取物、提取后留下的固体残渣以及未处理的龋坏和健康牙本质样本用硫酸-H₂O₂消化，然后通过对伯塞洛特颜色反应的特殊改进来分析氮含量。虽然链霉蛋白酶不侵蚀健康牙本质，但它溶解了龋坏牙本质中90%以上的氮。胶原酶溶解了约66%的氮，而次氯酸钠仅释放了龋坏牙本质中12%-20%的氮。在临床牙科中，龋坏牙本质的化学分解可能会减少对健康牙齿结构进行机械去除的需求。
