Matricon-Gondran M, Letocart M
Laboratoire d'Histologie et Cytologie des Invertébrés Marins, Université Pierre et Marie Curie, 12 rue Cuvier, Paris, 75005, France.
J Invertebr Pathol. 1999 Nov;74(3):224-34. doi: 10.1006/jipa.1999.4876.
We describe three distinct types of cells among Biomphalaria glabrata hemocytes: large cells with a tubulo-vesicular compartment, a component of the endocytic system, and with numerous mitochondria and large aggregates of glycogen particles; medium-size cells poor in organelles and glycogen; and small cells with organelles and few secretory granules. Other small hemocytes can be interpreted as juvenile cells. B. glabrata hemocytes contain few enzymes and do not show specific secretory granules, except for a subpopulation of large cells richer in acid phosphatase vesicles. Hemocytes have different aspects corresponding to different physiological states and their transitions: in quiescent hemocytes, the cell cortex is narrow and organelles are scattered in the cytoplasm, both in circulating cells characterized by thin-folded filopods and large macropinocytic vacuoles and in sedentary cells in which extended filopods connect to the extracellular matrix. In stress-activated hemocytes, the cortical region is thickened by polymerization of actin, and organelles are gathered around the nucleus. Fixed phagocytes are components of the connective tissue; the presence of numerous lysosomes and residual bodies and of acid phosphatase and peroxidase activities suggests a high phagocytic activity.
具有管状小泡区室（内吞系统的一个组成部分）、众多线粒体和大量糖原颗粒聚集体的大细胞；细胞器和糖原含量少的中等大小细胞；以及具有细胞器和少量分泌颗粒的小细胞。其他小血细胞可被解释为幼细胞。光滑双脐螺血细胞含有少量酶，除了富含酸性磷酸酶小泡的大细胞亚群外，不显示特定的分泌颗粒。血细胞根据不同的生理状态及其转变呈现出不同的形态：在静止的血细胞中，细胞皮层狭窄，细胞器分散在细胞质中，无论是以薄折叠丝状伪足和大型巨吞饮泡为特征的循环细胞，还是以延伸丝状伪足连接到细胞外基质的固定细胞。在应激激活的血细胞中，皮层区域因肌动蛋白聚合而增厚，细胞器聚集在细胞核周围。固定吞噬细胞是结缔组织的组成部分；大量溶酶体、残余体以及酸性磷酸酶和过氧化物酶活性的存在表明其具有高吞噬活性。
