视网膜色素上皮中视杆细胞外段的最终命运

Ultimate fate of rod outer segments in the retinal pigment epithelium.

作者信息

Thumann G, Bartz-Schmidt K U, Kociok N, Heimann K, Schraemeyer U

机构信息

Department of Vitreoretinal Surgery, University of Cologne, Germany.

出版信息

Pigment Cell Res. 1999 Oct;12(5):311-5. doi: 10.1111/j.1600-0749.1999.tb00764.x.

DOI:10.1111/j.1600-0749.1999.tb00764.x

PMID:10541040

Abstract

To investigate the degradation pathway of rod outer segments (ROS) in vivo, we injected gold-labeled ROS into the subretinal space of rabbits using a pars plana approach. Histology and electron microscopy performed on the specimens 72 hr after ROS injection revealed that the retina over the injection site was reattached, the retinal pigment epithelial (RPE) cells were intact, and gold granules were localized inside melanin granules and melanosomes. These results indicate that, in RPE, in vivo degradation of ROS is associated with melanosomes.

摘要

为了研究视杆细胞外段（ROS）在体内的降解途径，我们采用睫状体扁平部入路将金标记的ROS注入兔眼视网膜下间隙。在注入ROS 72小时后对标本进行组织学和电子显微镜检查，结果显示注射部位上方的视网膜已重新附着，视网膜色素上皮（RPE）细胞完整，金颗粒定位于黑色素颗粒和黑素小体内。这些结果表明，在RPE中，ROS在体内的降解与黑素小体有关。