Schraermeyer U, Stieve H
Institut für Biologie II (Zoologie), RWTH Aachen, Germany.
Cell Tissue Res. 1994 May;276(2):273-9. doi: 10.1007/BF00306113.
According to a recent hypothesis the melanin granules in the retinal pigment epithelium of mammals originate from photosensory membrane degradation. To test this hypothesis the retinal pigment epithelium of cattle was kept in tissue culture and exposed to gold-labelled rod outer segments. Gold granules were later detected inside phagosomes, melanosomes and mature melanin granules. Tyrosinase, the key enzyme in melanogenesis, was additionally localized inside phagosomes. These results indicate that in cultured retinal pigment epithelium the matrix of the melanosome can originate from phagosomes. Therefore, the melanosome is a specialized lysosome.
根据最近的一种假说，哺乳动物视网膜色素上皮中的黑色素颗粒源自光感受器膜的降解。为了验证这一假说，将牛的视网膜色素上皮进行组织培养，并使其暴露于金标记的视杆细胞外段。后来在吞噬体、黑素小体和成熟黑色素颗粒内部检测到了金颗粒。此外，黑色素生成中的关键酶酪氨酸酶也定位在吞噬体内部。这些结果表明，在培养的视网膜色素上皮中，黑素小体的基质可能源自吞噬体。因此，黑素小体是一种特殊的溶酶体。
