在增殖细胞中，于c-fos血清反应元件的ets盒上检测到的自主结合蛋白。
Autonomously binding protein detected on ets box of c-fos serum response element in proliferating cells.
作者信息
Masutani H, Magnaghi-Jaulin L, Groisman R, Ait-Si-Ali S, Robin P, Pritchard L L, Harel-Bellan A
机构信息
Laboratoire de Biologie des Tumeurs Humaines, CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.
出版信息
Gene Expr. 1999;8(1):33-42.
The serum response element (SRE) in the c-fos promoter contains an ets box whose integrity is required for full activation of this proto-oncogene by nerve growth factor (NGF) in PC12 rat pheochromocytoma cells. Electrophoretic mobility shift assays (EMSA) detect a protein in nuclear extracts that binds to the wild-type SRE, but not to an SRE containing a mutated ets box. Competition studies using unlabeled probes, and supershift experiments using antibodies and in vitro translated core serum response factor (SRF) indicate that the protein in question is not YY1, SAP-1, nor Elk-1 and that it does not exhibit ternary complex factor (TCF) activity, so that it may correspond to an autonomously binding Ets family protein. The complete disappearance of this "Ets-like autonomous binding factor" upon terminal differentiation of both L6alpha2 myoblastic and PC12 pheochromocytoma cells points to a possible role in the proliferation/differentiation process.
c-fos启动子中的血清反应元件（SRE）包含一个ets框，在PC12大鼠嗜铬细胞瘤细胞中，该框的完整性是神经生长因子（NGF）完全激活这个原癌基因所必需的。电泳迁移率变动分析（EMSA）检测到核提取物中有一种蛋白质能与野生型SRE结合，但不能与含有突变ets框的SRE结合。使用未标记探针的竞争研究，以及使用抗体和体外翻译的核心血清反应因子（SRF）的超迁移实验表明，所讨论的蛋白质不是YY1、SAP-1，也不是Elk-1，并且它不表现出三元复合因子（TCF）活性，因此它可能对应于一种自主结合的Ets家族蛋白。在L6α2成肌细胞和PC12嗜铬细胞瘤细胞终末分化时，这种“类Ets自主结合因子”完全消失，这表明它在增殖/分化过程中可能发挥作用。
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