一种识别小鼠乳腺肿瘤病毒长末端重复序列内远端负调控元件的蛋白质的鉴定。

Identification of a protein that recognizes a distal negative regulatory element within the mouse mammary tumor virus long terminal repeat.

作者信息

Kang C J, Peterson D O

机构信息

Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA.

出版信息

Virology. 1999 Nov 10;264(1):211-9. doi: 10.1006/viro.1999.9969.

DOI:10.1006/viro.1999.9969

PMID:10544147

Abstract

The mouse mammary tumor virus (MMTV) long terminal repeat contains a distal negative regulatory element (dNRE) that selectively represses activity of the proviral promoter in the absence of steroid hormone receptor-mediated activation. A protein, termed MMTV NRE-binding protein 1 (MNBP-1), that recognizes long terminal repeat sequences between -433 and -418 was identified by gel electrophoresis mobility shift assays and methylation interference footprinting in nuclear extracts of HeLa and Ltk(-) cells. Mutations within the defined binding site affect dNRE-mediated promoter repression in vivo. MNBP-1 has an apparent molecular mass of approximately 100 kDa as determined by gel filtration chromatography.

摘要

小鼠乳腺肿瘤病毒（MMTV）长末端重复序列包含一个远端负调控元件（dNRE），在缺乏类固醇激素受体介导的激活作用时，该元件可选择性抑制前病毒启动子的活性。通过凝胶电泳迁移率变动分析以及对HeLa和Ltk(-)细胞核提取物进行甲基化干扰足迹分析，鉴定出一种名为MMTV NRE结合蛋白1（MNBP-1）的蛋白质，它能识别-433至-418之间的长末端重复序列。在体内，限定结合位点内的突变会影响dNRE介导的启动子抑制作用。通过凝胶过滤色谱法测定，MNBP-1的表观分子量约为100 kDa。