Roudná V
Vet Med (Praha). 1979 Jan;24(1):59-64.
Two hundred and two samples of excrements were investigated for the presence of Toxoplasma gondii oocysts. Fifty-two samples were taken from dead domestic cats and a hundred and fifty samples were obtained from wild cats living in various localities of the CSSR. The investigation was orientative and a flotation method with the Breza flotation solution of the specific weight of 1.300 was used. If the finding was positive, residues of the excrements were floated with a saccharose, solution of the specific weight of 1.150 and containing 0.8% of phenol. The oocysts were rinsed several times, then they were sporulated in Petri dishes with water with a 2.5% solution of potassium dichromate. The sporulated oocysts, after rinsing, were injected i. p. to mice. The excrements of the fifty-two domestic cats were negative. Out of a hundred and fifty samples of the excrements of wild cats, one sample with the oocysts of isospore type was found; a biological test with mice proved the oocysts of Toxoplasma gondii. It may be inferred from the results obtained that the elimination of oocysts by cats is the same as given in foreign literature, and the occurrence rate will be about 2%.
对202份粪便样本进行了弓形虫卵囊检测。其中52份样本取自死亡的家猫，150份样本取自生活在捷克斯洛伐克社会主义共和国不同地区的野猫。此次调查具有探索性，采用了比重为1.300的布雷扎浮选液的浮选法。若检测结果为阳性，则将粪便残渣用比重为1.150且含有0.8%苯酚的蔗糖溶液浮选。卵囊经多次冲洗后，置于含有2.5%重铬酸钾溶液的培养皿中进行孢子化。孢子化后的卵囊冲洗后经腹腔注射给小鼠。52份家猫粪便检测结果为阴性。在150份野猫粪便样本中，发现一份等孢子类型的卵囊样本；对小鼠进行的生物学检测证实为弓形虫卵囊。从所得结果可以推断，猫排出卵囊的情况与国外文献报道一致，发生率约为2%。
