Heinemann H O, Pietra G G, Wagner M, Minda M
Virchows Arch B Cell Pathol. 1979 Feb 6;29(4):253-65. doi: 10.1007/BF02899358.
The effect of DL-2-bromopalmitate (BrPA), an analogue of palmitic acid (PA), on the utilization of this fatty acid by rat lungs was investigated by a combination of anatomic and biochemical methods. The experiments were performed in vitro on two types of preparations, isolated perfused lungs and lung slices. In the isolated lung preparation the substrate reached the lung via the capillaries, in lung slices via the alveolar epithelium. Electron microscope autoradiography showed that BrPA depressed uptake of PA by granular pneumocytes. Radioactivity recovered by tissue analysis and capture of CO2 established that PA oxidation and incorporation into phospholipids and triglycerides was depressed by BrPA. A close correlation was found between the reduction in radioactivity in phospholipids and the grain density over lamellar bodies. The study shows that BrPA reversibly interferes with the uptake and utilization of long chain fatty by granular pneumocytes. BrPA appears as a useful tool to study palmitate metabolism and surfactant production by the lung.
通过解剖学和生物化学方法相结合，研究了棕榈酸（PA）类似物DL-2-溴棕榈酸酯（BrPA）对大鼠肺脏利用这种脂肪酸的影响。实验在两种体外制备物上进行，即离体灌注肺和肺切片。在离体肺制备物中，底物通过毛细血管到达肺脏，在肺切片中则通过肺泡上皮细胞。电子显微镜放射自显影显示，BrPA抑制颗粒状肺细胞对PA的摄取。通过组织分析和二氧化碳捕获回收的放射性确定，BrPA抑制了PA的氧化以及其掺入磷脂和甘油三酯的过程。在磷脂放射性的降低与板层小体上的颗粒密度之间发现了密切的相关性。该研究表明，BrPA可逆地干扰颗粒状肺细胞对长链脂肪酸的摄取和利用。BrPA似乎是研究肺中棕榈酸代谢和表面活性剂产生的有用工具。
