Khar A, Ali A M, Begum Z, Pardhasaradhi B V, Varalakshmi C
Centre for Cellular and Molecular Biology, Hyderabad, India.
Indian J Biochem Biophys. 1999 Apr;36(2):77-81.
AK-5 tumour cells undergo apoptosis after treatment with rotenone an electron transport inhibitor and oligomycin which inhibits mitochondrial ATPases. Apoptotic process involves the induction of caspases 2 and 3, whereas caspase 1 does not seem to be participating in rotenone/oligomycin induced apoptosis. DEVD which is a specific inhibitor of caspase 3, inhibited apoptosis in AK-5 cells. We have also observed a significant lowering of intracellular pH in AK-5 cells which are induced into the apoptotic process by rotenone. These results suggest an important role for mitochondrial electron transport in the induction of apoptosis in AK-5 tumour cells.
用鱼藤酮（一种电子传递抑制剂）和寡霉素（一种抑制线粒体ATP酶的物质）处理AK-5肿瘤细胞后，这些细胞会发生凋亡。凋亡过程涉及半胱天冬酶2和3的诱导，而半胱天冬酶1似乎不参与鱼藤酮/寡霉素诱导的凋亡。DEVD是半胱天冬酶3的特异性抑制剂，它能抑制AK-5细胞的凋亡。我们还观察到，鱼藤酮诱导进入凋亡过程的AK-5细胞内的pH值显著降低。这些结果表明线粒体电子传递在AK-5肿瘤细胞凋亡诱导中起重要作用。
