Kamiusuki T, Monde T, Nakayama N, Yano K, Yoko T, Konakahara T
NEOS Company Ltd., Kohka, Shiga, 520-3213, Japan
J Colloid Interface Sci. 1999 Dec 1;220(1):123-127. doi: 10.1006/jcis.1999.6518.
Glassesand silica-gel packings modified with fluorocarbon and hydrocarbon silylation agents were prepared to investigate the separation mechanism of a fluorocarbon bonded layer in reversed-phase high-performance liquid chromatography (RP-HPLC). On the hydrocarbon layer the contact angles of benzene (Be) and hexafluorobenzene (FB) decreased with increasing carbon chain length of the modifier, and the mobile phase (MP) was independent of the modifier. The fluorocarbon layer gave a larger contact angle for each liquid than the hydrocarbon layer. The retention factor, k, of Be and FB on hydrocarbon packings increased with decreasing contact angle. It was demonstrated that the separation of Be and FB on hydrocarbon packings is caused by solvophobic interaction between solute molecules and the stationary layer. In contrast, k increased with increasing contact angle at the fluorocarbon layer. The retention of Be and FB on fluorocarbon packings may be attributed to the penetration of the solute molecules from the mobile phase into the fluorocarbon stationary layer. Copyright 1999 Academic Press.
制备了用氟碳和烃基硅烷化试剂改性的玻璃和硅胶填料，以研究反相高效液相色谱（RP-HPLC）中氟碳键合层的分离机理。在烃基层上，苯（Be）和六氟苯（FB）的接触角随改性剂碳链长度的增加而减小，且流动相（MP）与改性剂无关。对于每种液体，氟碳层的接触角都比烃基层大。Be和FB在烃基填料上的保留因子k随接触角的减小而增加。结果表明，Be和FB在烃基填料上的分离是由溶质分子与固定层之间的疏溶剂相互作用引起的。相反，在氟碳层上k随接触角的增加而增加。Be和FB在氟碳填料上的保留可能归因于溶质分子从流动相渗透到氟碳固定层中。版权所有1999年学术出版社。
