Gong G, Johnson M L, Barger-Lux M J, Heaney R P
Osteoporosis Research Center, Creighton University School of Medicine, Omaha, Nebraska, USA.
Osteoporos Int. 1999;9(4):307-11. doi: 10.1007/s001980050152.
We have recently shown that bone radiogrammetric dimensions are associated with vitamin D receptor gene polymorphism. Since parathyroid hormone (PTH) plays a central role in maintaining calcium homeostasis and in bone remodeling, we investigated whether bone radiogrammetric dimensions are associated with a PTH gene polymorphism in 91 healthy Caucasian women, who were premenopausal at entry into the study. These women had assessments of bone by radiogrammetry every five years for a median period of 20 years (range 4-27). DNA was extracted from white blood cells. A segment of the PTH gene with a polymorphism at a BstBI restriction site was amplified by polymerase chain reaction. Diameter, cortical thickness and cross-sectional area at standard sites of the metacarpals, radius and femur were measured with radiogrammetry. Higher metacarpal diameter and cross-sectional cortical area, and a slower decrease in radial cortical area with age, were associated with the absence of the BstBI restriction site of the PTH gene. PTH gene polymorphism accounts for about 7-9% of the total variances of bone dimensional variables. These findings suggest that the dimensions of long bones are influenced by allelic variations in the PTH gene or in genes nearby.
我们最近发现，骨放射测量尺寸与维生素D受体基因多态性相关。由于甲状旁腺激素（PTH）在维持钙稳态和骨重塑中起核心作用，我们调查了91名健康的白种女性（研究开始时处于绝经前）的骨放射测量尺寸是否与PTH基因多态性相关。这些女性每五年进行一次骨放射测量评估，中位时间为20年（范围4 - 27年）。从白细胞中提取DNA。通过聚合酶链反应扩增PTH基因中在BstBI限制性位点具有多态性的一段。用放射测量法测量掌骨、桡骨和股骨标准部位的直径、皮质厚度和横截面积。较高的掌骨直径和皮质横截面积，以及桡骨皮质面积随年龄增长下降较慢，与PTH基因的BstBI限制性位点缺失相关。PTH基因多态性约占骨尺寸变量总方差的7 - 9%。这些发现表明，长骨的尺寸受PTH基因或其附近基因的等位基因变异影响。
