Hou Y P, Jin Z M, Li Y B, Wu J, Walter H, Kido A, Prinz M
Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu 610041, Sichuan, P. R. China.
Int J Legal Med. 1999;112(6):400-2. doi: 10.1007/s004140050024.
In order to evaluate the forensic applicability of the STR locus D20S161 and construct a preliminary database, the genotype distributions and allele frequencies in five populations from three main ethnic groups were investigated, including Germans, Slovakians, African Americans, Japanese and Chinese. A total of 512 samples from unrelated individuals and 85 confirmed father/mother/ child triplets were analyzed by PCR and allele determination was carried out by comparison with a sequenced human allelic ladder. The results showed that D20S161 typing was both precise and reliable. A total of 7 alleles was found in these populations and no evidence of deviation from Hardy-Weinberg equilibrium was observed. Pairwise comparisons between populations showed that there were significant differences in the distributions of the allele frequencies among the three main ethnic groups. No mutation events were observed from the confirmed father/mother/child triplets. With a maximum likelihood method, the mutation rate was indirectly estimated as 2.5 x 10(-5). These results suggest that D20S161 is a useful marker for forensic casework and paternity analysis.
为了评估STR基因座D20S161在法医学中的适用性并构建初步数据库，我们调查了三个主要种族的五个群体中的基因型分布和等位基因频率，这些群体包括德国人、斯洛伐克人、非裔美国人、日本人以及中国人。通过聚合酶链反应（PCR）分析了总共512份无关个体的样本以及85份经确认的父亲/母亲/孩子三联体样本，并通过与测序的人类等位基因阶梯进行比较来确定等位基因。结果表明，D20S161分型既精确又可靠。在这些群体中总共发现了7个等位基因，未观察到偏离哈迪-温伯格平衡的证据。群体间的两两比较表明，三个主要种族群体中等位基因频率的分布存在显著差异。在经确认的父亲/母亲/孩子三联体中未观察到突变事件。采用最大似然法间接估计突变率为2.5×10⁻⁵。这些结果表明，D20S161是法医学案件工作和亲子鉴定分析中的一个有用标记。
