弧菌噬菌体KVP40和大肠杆菌噬菌体T4的基因组在编码主要衣壳蛋白的基因上游紧邻区域共享一个7千碱基对的同源区域。

Vibriophage KVP40 and coliphage T4 genomes share a homologous 7-kbp region immediately upstream of the gene encoding the major capsid protein.

作者信息

Matsuzaki S, Kuroda M, Kimura S, Tanaka S

机构信息

Department of Microbiology, Kochi Medical School, Oko, Nankoku, Kochi, Japan.

出版信息

Arch Virol. 1999;144(10):2007-12. doi: 10.1007/s007050050721.

DOI:10.1007/s007050050721

PMID:10550672

Abstract

Vibriophage KVP40, a large tailed DNA phage morphologically similar to T-even coliphages, has a major capsid protein (Mcp) homologous to the equivalent protein, gp23(*), of coliphage T4. The sequence analysis was extended to a 7-kbp region immediately upstream of the mcp gene encoding the precursor of Mcp. The region as a whole was fairly homologous to the corresponding region of the T4 genome and contained 8 ORFs homologous to T4 genes 17, 18, 19, 20, 67, 68, 21, and 22 in the same order as in T4. These findings thus strongly suggest that these two phages are phylogenetically related.

摘要

噬菌体KVP40是一种大型有尾DNA噬菌体，形态上与T偶数大肠杆菌噬菌体相似，其主要衣壳蛋白（Mcp）与大肠杆菌噬菌体T4的等效蛋白gp23(*)同源。序列分析扩展到编码Mcp前体的mcp基因上游紧邻的一个7kbp区域。该区域整体与T4基因组的相应区域相当同源，并包含8个与T4基因17、18、19、20、67、68、21和22同源的开放阅读框，顺序与T4中的相同。因此，这些发现有力地表明这两种噬菌体在系统发育上相关。