Nakashima N, Sasaki J, Toriyama S
National Institute of Sericultural and Entomological Science Owashi, Tsukuba, Ibaraki, Japan.
Arch Virol. 1999;144(10):2051-8. doi: 10.1007/s007050050726.
We determined the complete genome sequence of Himetobi P virus (HiPV), an insect picorna-like virus, which was isolated from the small brown planthopper, Laodelphax striatellus. The genome of HiPV consists of 9,275 nucleotides excluding the poly (A) tail, and contains two large open reading frames (ORFs), which were separated by a 176-nucleotide noncoding region. The deduced amino acid sequence of the first ORF contains core motifs of picornaviral helicase, protease, and RNA-dependent RNA polymerase. The capsid protein-coding region was mapped onto the second ORF by determining the N-terminal amino acid sequences of the capsid proteins. Subgenomic RNA for the capsid protein gene was not detected in the infected tissue. The capsid protein precursor gene of HiPV lacks an AUG initiation codon at the expected position and the upstream sequence of the gene is predicted to form several stem-loop structures, suggesting that the precursor is produced by internal ribosome entry site (IRES) mediated-translation, as occurs in Plutia stali intestine virus (PSIV). These characteristics of the HiPV genome are similar to those of a new group of RNA viruses consisting of Drosophila C virus (DCV), Rhopalosiphum padi virus (RhPV), and PSIV.
我们测定了从灰飞虱（Laodelphax striatellus）中分离得到的一种昆虫类微小核糖核酸病毒——希梅托比P病毒（Himetobi P virus，HiPV）的全基因组序列。HiPV的基因组由9275个核苷酸组成（不包括聚腺苷酸尾），包含两个大的开放阅读框（ORF），它们被一个176个核苷酸的非编码区隔开。第一个ORF推导的氨基酸序列包含微小核糖核酸病毒解旋酶、蛋白酶和RNA依赖性RNA聚合酶的核心基序。通过确定衣壳蛋白的N端氨基酸序列，将衣壳蛋白编码区定位到第二个ORF上。在感染组织中未检测到衣壳蛋白基因的亚基因组RNA。HiPV的衣壳蛋白前体基因在预期位置缺少AUG起始密码子，并且该基因的上游序列预计会形成几个茎环结构，这表明该前体是由内部核糖体进入位点（IRES）介导的翻译产生的，就像在斯氏果蝇肠道病毒（Plutia stali intestine virus，PSIV）中那样。HiPV基因组的这些特征与由果蝇C病毒（Drosophila C virus，DCV）、禾谷缢管蚜病毒（Rhopalosiphum padi virus，RhPV）和PSIV组成的一组新的RNA病毒相似。
