Rowe G E, Margaritis A, Lan Q, Bassi A S, Zhu J X
Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.
Biotechnol Bioeng. 1999 Dec 20;65(6):613-21.
The kinetics of adsorption of bovine serum albumin on an anion-exchange resin were measured in a batch system using a flow cell and ultraviolet absorbance, as a function of initial liquid-phase protein concentration and solid-to-liquid phase ratio. A new mathematical model for adsorption kinetics is presented that fits the experimental data to give a highly linear relationship with time, following a short transient period. Numerical integration of the differential form of the new composite nonlinear (CNL) kinetic model, containing three independent parameters, is shown to describe the dynamics of batch adsorption much better than alternative lumped parameter models. Although the new model is phenomenological rather than mechanistic, its principal parameter is shown to be a direct linear function of a physically measurable quantity. This study demonstrates that the model can accurately simulate protein concentration-time profiles using parameter estimates derived from correlations over a wide range of initial protein concentrations and phase ratios. The new CNL model is shown to be considerably superior to the Langmuir and solid-film linear kinetic models in this regard, having the additional advantage that an equilibrium isotherm for the system is not required.
在间歇系统中，使用流通池和紫外吸光度，测量了牛血清白蛋白在阴离子交换树脂上的吸附动力学，该动力学是初始液相蛋白质浓度和固液相比的函数。提出了一种新的吸附动力学数学模型，该模型能使实验数据在短暂的瞬态期后与时间呈现高度线性关系。含有三个独立参数的新型复合非线性（CNL）动力学模型的微分形式的数值积分表明，它比其他集总参数模型能更好地描述间歇吸附动力学。虽然新模型是唯象的而非机理的，但其主要参数被证明是一个可物理测量量的直接线性函数。本研究表明，该模型可以使用从广泛的初始蛋白质浓度和相比的相关性得出的参数估计值，准确模拟蛋白质浓度-时间曲线。在这方面，新的CNL模型被证明明显优于朗缪尔模型和固膜线性动力学模型，还有一个额外的优点是不需要该系统的平衡等温线。
