产酸克雷伯菌p2对淀粉的发酵，该菌含有携带α-淀粉酶和支链淀粉酶基因的质粒。
Fermentation of starch by Klebsiella oxytoca p2, containing plasmids with alpha-amylase and pullulanase genes.
作者信息
dos Santos V L, Araújo E F, de Barros E G, Guimarães W V
机构信息
Departamento de Microbiologia and Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa MG, 36571-000, Brazil.
出版信息
Biotechnol Bioeng. 1999 Dec 20;65(6):673-6. doi: 10.1002/(sici)1097-0290(19991220)65:6<673::aid-bit8>3.0.co;2-j.
Klebsiella oxytoca P2(pC46), an ethanol-producing recombinant, has been evaluated in fermentation of maltose and starch. The maximum ethanol produced by P2(pC46) was 0.34 g ethanol/g maltose and 0.38, 0.40, or 0.36 g ethanol/g starch in fermentation of 1, 2, or 4% starch, representing 68, 71, and 64% the theoretical yield. The pC46 plasmid transformed to cells of K. oxytoca P2 reduced the ethanol production from maltose and starch. In fermentation of starch after its digestion at 60 degrees C for 24 h, in two-step fermentation, the time for maximum ethanol production was reduced to 12-24 h and the theoretical yield was around 90%. The increase in starch concentration resulted in lower alpha-amylase activity but in higher pullulanase activity. The high activity and thermostability of the amylolytic enzymes from this transformant suggest that it has a potential for amylolytic enzymes source.
产酸克雷伯菌P2(pC46)是一种产乙醇的重组菌，已在麦芽糖和淀粉发酵中进行了评估。P2(pC46)在发酵中产生的最大乙醇量为0.34克乙醇/克麦芽糖，在1%、2%或4%淀粉发酵中分别为0.38、0.40或0.36克乙醇/克淀粉，分别代表理论产量的68%、71%和64%。转入产酸克雷伯菌P2细胞的pC46质粒降低了麦芽糖和淀粉的乙醇产量。在60℃消化24小时后的淀粉发酵中，两步发酵时，最大乙醇产量的时间缩短至12 - 24小时，理论产量约为90%。淀粉浓度的增加导致α-淀粉酶活性降低，但支链淀粉酶活性升高。该转化体的淀粉分解酶的高活性和热稳定性表明它有作为淀粉分解酶来源的潜力。
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