一种针对远缘链球菌的DNA探针。

A DNA probe specific to Streptococcus sobrinus.

作者信息

Ida H, Igarashi T, Yamamoto A, Goto N, Sasa R

机构信息

Department of Pediatric Dentistry, Showa University School of Dentistry, Tokyo, Japan.

出版信息

Oral Microbiol Immunol. 1999 Aug;14(4):233-7. doi: 10.1034/j.1399-302x.1999.140406.x.

DOI:10.1034/j.1399-302x.1999.140406.x

PMID:10551167

Abstract

Three DNA fragments (SSB-1, -2 and -3) in the dextranase gene (dex) of Streptococcus sobrinus were amplified by polymerase chain reaction and used as DNA probes. The probes were examined for the specificity and the sensitivity of hybridization with DNA of oral streptococcal species. While probes SSB-1 and SSB-2 were specific to both S. sobrinus and Streptococcus downei, SSB-3 was specific only to S. sobrinus. SSB-3 was able to detect 5 ng of chromosomal DNA purified from S. sobrinus NIDR6715 and DNA extracted from 1 x 10(5) cells of the strain. In addition, SSB-3 could differentiate clinical isolates of S. sobrinus from Streptococcus mutans. These results suggest that SSB-3 is an effective DNA-probe to detect and to identify S. sobrinus.

摘要

通过聚合酶链反应扩增了远缘链球菌葡聚糖酶基因（dex）中的三个DNA片段（SSB - 1、- 2和- 3），并将其用作DNA探针。检测了这些探针与口腔链球菌属DNA杂交的特异性和敏感性。虽然探针SSB - 1和SSB - 2对远缘链球菌和唐氏链球菌均具有特异性，但SSB - 3仅对远缘链球菌具有特异性。SSB - 3能够检测到从远缘链球菌NIDR6715纯化的5 ng染色体DNA以及从该菌株的1×10⁵个细胞中提取的DNA。此外，SSB - 3能够区分远缘链球菌的临床分离株与变形链球菌。这些结果表明，SSB - 3是检测和鉴定远缘链球菌的有效DNA探针。