Igarashi T, Asaga E, Murai C, Sasa R, Goto N
Departments of Oral Microbiology Pediatric Dentistry, Showa University School of Dentistry, Tokyo, Japan.
Lett Appl Microbiol. 2004;38(2):125-9. doi: 10.1111/j.1472-765x.2003.01451.x.
To establish a rapid method to differentiate Streptococcus downei and S. sobrinus by multiplex PCR.
A PCR primer pair specific to S. downei was designed on the basis of the nucleotide sequence of the dextranase gene of S. downei NCTC 11391T. The primer pair specifically detected S. downei, but none of the other mutans streptococci (16 strains of six species). The PCR procedure was capable of detecting 1 pg of genomic DNA purified from S. downei NCTC 11391 and as few as 14 CFU of S. downei cells. The mixture of primer pairs specific to each S. downei (this study) and S. sobrinus (Igarashi et al. 2000) detected only the strains of these two species among all the mutans streptococcal strains, and concomitantly differentiated the two species by species-specific amplicons of different lengths.
The present PCR method is highly specific to S. downei and is useful for detection and identification of S. downei.
Multiplex PCR using dextranase gene primers is a useful method for simultaneous detection and differentiation of S. downei and S. sobrinus.
建立一种通过多重PCR鉴别唐氏链球菌和远缘链球菌的快速方法。
根据唐氏链球菌NCTC 11391T葡聚糖酶基因的核苷酸序列设计了一对特异于唐氏链球菌的PCR引物。该引物对能特异性检测唐氏链球菌，而不能检测其他变形链球菌（6个种的16株菌）。该PCR程序能够检测从唐氏链球菌NCTC 11391中纯化的1 pg基因组DNA，以及低至14 CFU的唐氏链球菌细胞。特异于唐氏链球菌（本研究）和远缘链球菌（Igarashi等人，2000年）的引物对混合物在所有变形链球菌菌株中仅检测到这两个种的菌株，并通过不同长度的种特异性扩增子同时鉴别这两个种。
本PCR方法对唐氏链球菌具有高度特异性，可用于唐氏链球菌的检测和鉴定。
使用葡聚糖酶基因引物的多重PCR是同时检测和鉴别唐氏链球菌和远缘链球菌的有用方法。
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