Drabent B, Kim J S, Albig W, Prats E, Cornudella L, Doenecke D
Institute of Biochemistry and Molecular Cell Biology, Department of Molecular Biology, Georg-August-University Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany.
J Mol Evol. 1999 Nov;49(5):645-55. doi: 10.1007/pl00006585.
We isolated five different phage clones containing histone gene clusters with up to five H1 genes per phage clone from a Mytilus edulis genomic library. Among these H1 genes, nine gene types coding for five different H1 proteins have been identified. All H1 histone genes were located on repetitive restriction fragments with only slightly different sizes. The H1 coding regions show highly related sequences, suggesting that the multitude of H1 genes has evolved by gene duplication events. Core histone genes could not be found on these five Mytilus edulis genome fragments.
我们从紫贻贝基因组文库中分离出五个不同的噬菌体克隆，每个噬菌体克隆含有多达五个H1基因的组蛋白基因簇。在这些H1基因中，已鉴定出编码五种不同H1蛋白的九种基因类型。所有H1组蛋白基因都位于大小仅略有不同的重复限制性片段上。H1编码区显示出高度相关的序列，这表明众多的H1基因是通过基因复制事件进化而来的。在这五个紫贻贝基因组片段上未发现核心组蛋白基因。
