Albig Werner, Warthorst Ursula, Drabent Birgit, Prats Eva, Cornudella Luis, Doenecke Detlef
Institut für Biochemie und Molekulare Zellbiologie, Universität Göttingen, Humboldtallee 23, Germany.
J Mol Evol. 2003 May;56(5):597-606. doi: 10.1007/s00239-002-2428-8.
Comparison of histone gene cluster arrangements in several species has revealed a broad spectrum of histone gene patterns. To elucidate the core histone gene organization in a mollusk, we have analyzed a Mytilus edulis genomic library and have isolated eight phage clones containing core histone genes. Analysis of insert DNA revealed that the core histone genes are arranged as regular gene repeats of all four core histones. The repeats do not contain linker histone genes. The clones are distributed into two groups of dissimilar repeated units with a common size of about 5.6 kb. The genes of each core histone class in the distinct repeats encode identical histone proteins and have comparable gene arrangements in the two repeat units. However, the intergenic sequences differ significantly. The core histone genes are organized as large clusters of about 100 repeats each. Previously, we have shown that the linker histone genes in M. edulis are also organized in a cluster of repeats of solitary H1 genes. Hence, this is the first case of a separate, clustered organization of both core and linker histone genes, respectively.
对多个物种的组蛋白基因簇排列进行比较，揭示了广泛的组蛋白基因模式。为了阐明软体动物中的核心组蛋白基因组织，我们分析了紫贻贝基因组文库，并分离出八个含有核心组蛋白基因的噬菌体克隆。对插入DNA的分析表明，核心组蛋白基因以所有四种核心组蛋白的规则基因重复形式排列。这些重复序列不包含连接组蛋白基因。这些克隆被分为两组不同的重复单元，共同大小约为5.6 kb。不同重复序列中每个核心组蛋白类别的基因编码相同的组蛋白，并在两个重复单元中具有可比的基因排列。然而，基因间序列差异显著。核心组蛋白基因被组织成大约100个重复序列的大簇。此前，我们已经表明紫贻贝中的连接组蛋白基因也以单独的H1基因重复簇的形式组织。因此，这是分别存在核心组蛋白基因和连接组蛋白基因单独成簇组织的首例。
