通过电子自旋共振法利用H(2)O(2)/NaOH/DMSO体系评估抗氧化剂的自由基清除活性。

Evaluation of free radical scavenging activities of antioxidants with an H(2)O(2)/NaOH/DMSO system by electron spin resonance.

作者信息

Yoshimura Y, Inomata T, Nakazawa H, Kubo H, Yamaguchi F, Ariga T

机构信息

Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan.

出版信息

J Agric Food Chem. 1999 Nov;47(11):4653-6. doi: 10.1021/jf990422w.

DOI:10.1021/jf990422w

PMID:10552866

Abstract

An H(2)O(2)/NaOH/DMSO system has been developed for the formation of three free radicals, and the application of the system was examined with the antioxidants ascorbic acid and tocopherol. Superoxide anion, hydroxyl radical,and methyl radical are simultaneously generated in this system. The scavenging activity of ascorbic acid and tocopherol for these radicals was estimated by 5, 5'-dimethyl-1-pyrroline-N-oxide spin trapping electron spin resonance. Both water-soluble and oil-soluble antioxidants could be evaluated by using this system. Ascorbic acid specifically inhibited the superoxide anion and hydroxyl radical, whereas tocopherol suppressed the methyl radical.

摘要

已开发出一种用于生成三种自由基的H₂O₂/NaOH/DMSO体系，并以抗氧化剂抗坏血酸和生育酚对该体系的应用进行了研究。在该体系中同时产生超氧阴离子、羟基自由基和甲基自由基。通过5,5'-二甲基-1-吡咯啉-N-氧化物自旋捕获电子自旋共振估算抗坏血酸和生育酚对这些自由基的清除活性。使用该体系可评估水溶性和油溶性抗氧化剂。抗坏血酸特异性抑制超氧阴离子和羟基自由基，而生育酚抑制甲基自由基。