一种基于高效液相色谱法的N-酰基谷氨酸水解通用检测方法。
A general high-performance liquid chromatography-based assay for the hydrolysis of N-acyl glutamates.
作者信息
Myers R S, Paton P T, Dastgah A, Martell J R, Berkman C E
机构信息
Department of Chemistry and Biochemistry, San Francisco State University, 1600 Holloway Avenue, San Francisco, California 94132, USA.
出版信息
Anal Biochem. 1999 Nov 15;275(2):187-91. doi: 10.1006/abio.1999.4327.
A rapid and simple HPLC assay has been developed to separate and quantify N-acyl glutamates and the corresponding carboxylic acids of the acyl moiety. This method was specifically developed to assay hydrolytic activity for glutamate carboxypeptidases. Although established assays for specific substrates of such enzymes exist, they may not be amenable for examining the hydrolytic activity of new substrate probes. This assay was developed to accommodate such probes.
已开发出一种快速简便的高效液相色谱（HPLC）分析法，用于分离和定量N - 酰基谷氨酸及其相应的酰基部分羧酸。该方法专门用于测定谷氨酸羧肽酶的水解活性。尽管存在针对此类酶特定底物的既定分析方法，但它们可能不适用于检测新底物探针的水解活性。开发此分析方法是为了适应此类探针。
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