Investigation of the hydrolytic decomposition of 5-iodine-2'-desoxyuridine by high-performance liquid chromatography.

A new HPLC method was developed for the monitoring of the hydrolytic decomposition of 5-iodine-2'-desoxyuridine in aqueous solution and for the stability assay of this compound in formulation. The separation of 5-iodine-2'-desoxyuridine and its decomposition products (5-iodineuracil and uracil) was performed on a u Bondapack C18 column using a mixture of water and methanol as eluent. On the basis of the experimental data, the kinetic order and the rate-constants of both consecutive reactions were determined.