[牛晶状体膜蛋白免疫反应诱导的大鼠晶状体上皮细胞损伤与白内障形成]

[Rat lens epithelial cell damage and cataract formation induced by immunological response to bovine lens membrane protein].

作者信息

Tanemoto K, Sueno T, Obazawa H, Shinohara T, Akatsuka A

机构信息

Department of Ophthalmology, Tokai University School of Medicine, Isehara, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1999 Oct;103(10):713-21.

PMID:10554545

Abstract

PURPOSE

To evaluate the correlation of cataractogenesis and immune mechanisms, we investigated the rat lens morphologically and immunologically.

METHODS AND RESULTS

Wistar rats were divided into three groups: Group A was immunized with bovine-lens membrane protein (B-LMP) and adjuvant, Group B was immunized with adjuvant only, and Group C was not given any treatment as a control. Titer levels of anti-B-LMP antibody and anti-Rat-LMP antibody were elevated and posterior subcapsular cataract was developed in Group A. In flat preparations, a noncellular part in the lens epithelium was observed in all members of Group A. In this noncellular part, a lens capsule protruding into the lens epithelial cell layer was observed by light microscopy.

CONCLUSION

These data suggest that lens epithelial cells may be damaged by immune response, causing the development of cataract.

摘要

目的

为了评估白内障形成与免疫机制之间的相关性，我们对大鼠晶状体进行了形态学和免疫学研究。

方法与结果

将Wistar大鼠分为三组：A组用牛晶状体膜蛋白（B-LMP）和佐剂免疫，B组仅用佐剂免疫，C组不做任何处理作为对照。A组抗B-LMP抗体和抗大鼠LMP抗体的滴度水平升高，并出现后囊下白内障。在扁平制片中，A组所有大鼠的晶状体上皮中均观察到无细胞区。在这个无细胞区，通过光学显微镜观察到晶状体囊膜突入晶状体上皮细胞层。