Zhu J, Nathan C, Ding A
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, USA.
Biochim Biophys Acta. 1999 Sep 21;1451(2-3):219-23. doi: 10.1016/s0167-4889(99)00111-1.
Expression of secretory leukocyte protease inhibitor (SLPI) suppresses the ability of macrophages to respond to bacterial lipopolysaccharide (LPS). Here, addition of recombinant or native SLPI to the extracellular medium was non-suppressive, while transfection with a non-secretory form of SLPI was fully suppressive, an effect overcome by treatment with interferon-gamma. A portion of the SLPI produced by untransfected macrophages was localized in the cytosol. Thus, SLPI can act intracellularly to block macrophage activation by LPS.
分泌型白细胞蛋白酶抑制剂（SLPI）的表达可抑制巨噬细胞对细菌脂多糖（LPS）作出反应的能力。在此，向细胞外培养基中添加重组或天然SLPI并无抑制作用，而用非分泌形式的SLPI进行转染则具有完全抑制作用，这种作用可被γ干扰素处理所克服。未转染的巨噬细胞产生的一部分SLPI定位于细胞质中。因此，SLPI可在细胞内发挥作用，阻断LPS对巨噬细胞的激活。
