Taffe M A, Weed M R, Gold L H
Department of Neuropharmacology, CVN-7, The Scripps Research Institute, La Jolla, CA 92037, USA.
Brain Res Cogn Brain Res. 1999 Oct 25;8(3):203-12. doi: 10.1016/s0926-6410(99)00021-x.
Rhesus monkeys (6) were trained on a test battery including cognitive tests adapted from a human neuropsychological assessment battery (CANTAB; CeNeS, Cambridge, UK) as well as a bimanual motor skill task. The complete battery included tests of memory (delayed non-match to sample, DNMS; self-ordered spatial search, SOSS), reaction time (RT), motivation (progressive ratio; PR) and fine motor coordination (bimanual). The animals were trained to asymptotic performance in all tasks and then were administered two of the four CANTAB tasks on alternate weekdays (PR/SWM; DNMS/RT) with the bimanual task being administered on each weekday. The effect of acute administration of scopolamine (3-24 microg/kg, i.m.) on performance was then determined. Although performance on DNMS was impaired there was no interaction of drug treatment with retention interval, suggesting that scopolamine does not increase the rate of forgetting in this task. Scopolamine administration produced a decrement in SOSS performance that was dependent on task difficulty as well as dose. Scopolamine also impaired motor responses, resulting in increased time required to complete the bimanual motor task and increased movement time in the RT task. Performance in the PR task was decreased in a dose-dependent fashion by scopolamine. The results suggest that scopolamine interferes with memory storage and motor responses but not memory retention/retrieval or vigilance. The findings demonstrate that the test battery is useful for distinguishing the effects of neuropharmacological manipulation on various aspects of cognitive performance in monkeys.
对6只恒河猴进行了一系列测试训练，其中包括借鉴人类神经心理学评估组套（CANTAB；CeNeS，英国剑桥）改编的认知测试以及一项双手运动技能任务。完整的组套包括记忆测试（延迟非匹配样本，DNMS；自我排序空间搜索，SOSS）、反应时间（RT）、动机（渐进比率；PR）和精细运动协调（双手）测试。让这些动物在所有任务中训练至渐近表现，然后在工作日交替进行四项CANTAB任务中的两项（PR/SWM；DNMS/RT），且每个工作日都进行双手任务。随后确定了急性注射东莨菪碱（3 - 24微克/千克，肌肉注射）对表现的影响。尽管DNMS任务的表现受损，但药物治疗与记忆间隔之间没有相互作用，这表明东莨菪碱不会增加该任务中的遗忘率。东莨菪碱给药导致SOSS表现下降，这取决于任务难度和剂量。东莨菪碱还损害了运动反应，导致完成双手运动任务所需时间增加以及RT任务中的运动时间增加。东莨菪碱使PR任务的表现以剂量依赖的方式下降。结果表明，东莨菪碱干扰记忆存储和运动反应，但不影响记忆保持/检索或警觉性。这些发现表明该测试组套有助于区分神经药理学操作对猴子认知表现各个方面的影响。
