鱼类促性腺激素受体的双重性：马苏大麻哈鱼（Oncorhynchus rhodurus）卵巢和睾丸中表达的第二种促性腺激素受体cDNA的克隆与功能特性分析
The duality of fish gonadotropin receptors: cloning and functional characterization of a second gonadotropin receptor cDNA expressed in the ovary and testis of amago salmon (Oncorhynchus rhodurus).
作者信息
Oba Y, Hirai T, Yoshiura Y, Yoshikuni M, Kawauchi H, Nagahama Y
机构信息
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, 444-8585, Japan.
出版信息
Biochem Biophys Res Commun. 1999 Nov 19;265(2):366-71. doi: 10.1006/bbrc.1999.1700.
We previously isolated a cDNA encoding a gonadotropin receptor (sGTH-R) from amago salmon (Oncorhynchus rhodurus) ovarian follicles. In the present study, we cloned a second gonadotropin receptor (sGTH-RI) from the same RNA preparations. Overall sequence homology between sGTH-RI and sGTH-R is 44%. The highest homology occurs with mammalian FSH receptors (49%). Functional characterization examined in COS-7 cells transiently transfected with sGTH-RI showed the largest increase in cAMP production when exposed to salmon GTH I. These results provide the first evidence in any ectothermic vertebrate, the amago salmon, of the duality of gonadotropin receptors.
我们之前从秋鲑（Oncorhynchus rhodurus）卵巢卵泡中分离出一个编码促性腺激素受体（sGTH-R）的cDNA。在本研究中，我们从相同的RNA制剂中克隆出了第二个促性腺激素受体（sGTH-RI）。sGTH-RI与sGTH-R之间的总体序列同源性为44%。与哺乳动物促卵泡激素受体的同源性最高（49%）。在用sGTH-RI瞬时转染的COS-7细胞中进行的功能特性研究表明，当暴露于鲑鱼促性腺激素I时，细胞内环磷酸腺苷（cAMP）产量增加幅度最大。这些结果首次在任何变温脊椎动物——秋鲑中证明了促性腺激素受体的双重性。
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