Rocha Ana, Gómez Ana, Zanuy Silvia, Cerdá-Reverter José Miguel, Carrillo Manuel
Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones Cientificas, 12595 Torre la Sal, Ribera de Cabanes, Castellón, Spain.
Mol Cell Endocrinol. 2007 Jun 30;272(1-2):63-76. doi: 10.1016/j.mce.2007.04.007. Epub 2007 May 4.
The follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) play central roles in vertebrate reproduction. They act through their cognate receptors to stimulate testicular and ovarian functions. The present study reports the cloning and characterization of two sea bass (Dicentrarchus labrax) cDNAs encoding a FSH receptor (sbsFSHR) and a LH receptor (sbsLHR). The mature proteins display typical features of the glycoprotein hormone receptor family members, but the sbsFSHR also contains some remarkable differences when compared with other fish or mammalian FSHRs. Among them, a distinct extracellular N-terminal cysteine domain as regards to its length and cysteine number, and the presence of an extra leucine-rich repeat. Expression analysis revealed that the sbsFSHR is exclusively expressed in gonadal tissues, specifically in the follicular wall of previtellogenic and early-vitellogenic follicles. On the contrary, sbsLHR mRNA was found to be widely distributed in sea bass somatic tissues. When stably expressed in mammalian cell lines, sbsFSHR was specifically stimulated by bovine FSH, while sbsLHR was activated by both bovine LH and FSH. Nevertheless, specific stimulation of the sbsLHR was observed when recombinant sea bass gonadotropins were used. The isolation of a FSHR and a LHR in sea bass opens new ways to study gonadotropin action in this species.
促卵泡激素（FSH）和促黄体生成素（LH）在脊椎动物繁殖中起着核心作用。它们通过其同源受体发挥作用，刺激睾丸和卵巢功能。本研究报告了两条海鲈（欧洲鲈）cDNA的克隆和特征，这两条cDNA分别编码促卵泡激素受体（sbsFSHR）和促黄体生成素受体（sbsLHR）。成熟蛋白显示出糖蛋白激素受体家族成员的典型特征，但与其他鱼类或哺乳动物的促卵泡激素受体相比，sbsFSHR也存在一些显著差异。其中，在细胞外N端半胱氨酸结构域的长度和半胱氨酸数量方面有明显差异，并且存在一个额外的富含亮氨酸的重复序列。表达分析表明，sbsFSHR仅在性腺组织中表达，特别是在卵黄生成前期和卵黄生成早期卵泡的卵泡壁中。相反，发现sbsLHR mRNA广泛分布于海鲈的体细胞组织中。当在哺乳动物细胞系中稳定表达时，sbsFSHR受到牛促卵泡激素的特异性刺激，而sbsLHR则被牛促黄体生成素和促卵泡激素激活。然而，当使用重组海鲈促性腺激素时，观察到了对sbsLHR的特异性刺激。海鲈中促卵泡激素受体和促黄体生成素受体的分离为研究该物种中促性腺激素的作用开辟了新途径。
