Inagaki H, Maeda S, Lin K H, Shimizu N, Saito T
Department of Chemistry, National Industrial Research Institute of Nagoya, 1-1 Hirate-cho, Kita-ku, Nagoya, 462-8510, Japan.
Biochem Biophys Res Commun. 1999 Nov 19;265(2):462-8. doi: 10.1006/bbrc.1999.1723.
Drosophila DLG (Discs Large Tumor Suppresser Protein) is a component of septate junctions, and disruption of its gene leads to over growth of imaginal discs. Homologs of Drosophila DLG recently isolated from mammalian tissue have been classified as members of the MAGUK (Membrane Associated GUanylate Kinase) superfamily of proteins. Using a modified RT-PCR method applied to rat tissues, we have isolated cDNA clones encoding a novel MAGUK family member that we have named rDLG6. Immunoblot and immunohistochemical analyses revealed that rDLG6 protein is predominantly expressed in brain. GST pull-down assays showed that the PDZ domain of rDLG6 protein binds to the C-terminus of the AMPA (alpha-Amino-3-hydroxy-5-Methyl-isoxazole-4-Propionic Acid) receptor GluR2 subunit.
果蝇盘状大肿瘤抑制蛋白（Drosophila DLG）是分隔连接的一个组成部分，其基因的破坏会导致成虫盘过度生长。最近从哺乳动物组织中分离出的果蝇DLG同源物已被归类为膜相关鸟苷酸激酶（MAGUK）蛋白超家族的成员。通过应用于大鼠组织的改良逆转录聚合酶链反应（RT-PCR）方法，我们分离出了编码一种新型MAGUK家族成员的cDNA克隆，我们将其命名为rDLG6。免疫印迹和免疫组织化学分析表明，rDLG6蛋白主要在脑中表达。谷胱甘肽-S-转移酶（GST）下拉实验表明，rDLG6蛋白的PDZ结构域与α-氨基-3-羟基-5-甲基异恶唑-4-丙酸（AMPA）受体GluR2亚基的C末端结合。
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