Chiba Y, Ishikawa M, Kijima F, Tyson R H, Kim J, Yamamoto A, Nambara E, Leustek T, Wallsgrove R M, Naito S
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Science. 1999 Nov 12;286(5443):1371-4. doi: 10.1126/science.286.5443.1371.
Control of messenger RNA (mRNA) stability serves as an important mechanism for regulating gene expression. Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Transfection experiments with wild-type and mutant forms of the CGS gene suggest that an amino acid sequence encoded by the first exon of CGS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.
信使核糖核酸（mRNA）稳定性的调控是调节基因表达的重要机制。对可溶性甲硫氨酸（Met）过度积累的拟南芥突变体进行分析发现，甲硫氨酸生物合成中的关键酶——胱硫醚γ-合酶（CGS）的基因在mRNA稳定性水平受到调控。用CGS基因的野生型和突变型进行转染实验表明，CGS第一个外显子编码的氨基酸序列以顺式作用使自身mRNA不稳定，这一过程由Met或其一种代谢产物激活。
