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Transcriptional analysis of the rubrerythrin and superoxide dismutase genes of Clostridium perfringens.

作者信息

Geissmann T A, Teuber M, Meile L

机构信息

Laboratorium für Lebensmittel-Mikrobiologie, Institut für Lebensmittelwissenschaft, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland.

出版信息

J Bacteriol. 1999 Nov;181(22):7136-9. doi: 10.1128/JB.181.22.7136-7139.1999.

Abstract

We cloned and sequenced a 2.7-kb fragment of chromosomal DNA from Clostridium perfringens containing the superoxide dismutase-encoding gene, sod. Previously, rubrerythrin from C. perfringens had been isolated and its gene (rbr) had been cloned (Y. Lehmann, L. Meile, and M. Teuber, J. Bacteriol. 178:7152-7158, 1996). Northern blot experiments revealed a length of approximately 800 bases for each transcript of rbr and sod of C. perfringens. Thus, rbr and sod each represent a monocistronic operon. Their transcription start points were located by primer extension analyses. sod transcription was shown to depend on the growth phase, and it reached a maximum during the transition from log phase to stationary phase. Neither sod nor rbr transcription was influenced by oxidative stress.

摘要

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