Geissmann T A, Teuber M, Meile L
Laboratorium für Lebensmittel-Mikrobiologie, Institut für Lebensmittelwissenschaft, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland.
J Bacteriol. 1999 Nov;181(22):7136-9. doi: 10.1128/JB.181.22.7136-7139.1999.
We cloned and sequenced a 2.7-kb fragment of chromosomal DNA from Clostridium perfringens containing the superoxide dismutase-encoding gene, sod. Previously, rubrerythrin from C. perfringens had been isolated and its gene (rbr) had been cloned (Y. Lehmann, L. Meile, and M. Teuber, J. Bacteriol. 178:7152-7158, 1996). Northern blot experiments revealed a length of approximately 800 bases for each transcript of rbr and sod of C. perfringens. Thus, rbr and sod each represent a monocistronic operon. Their transcription start points were located by primer extension analyses. sod transcription was shown to depend on the growth phase, and it reached a maximum during the transition from log phase to stationary phase. Neither sod nor rbr transcription was influenced by oxidative stress.