Meile L, Fischer K, Leisinger T
Institut für Lebensmittelwissenschaften, Eidgenössiche Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.
FEMS Microbiol Lett. 1995 May 15;128(3):247-53. doi: 10.1111/j.1574-6968.1995.tb07532.x.
A gene (sod) encoding superoxide dismutase (SOD) was isolated from the strictly anaerobic archaeon Methanobacterium thermoautotrophicum Marburg. Its identify was confirmed by functional complementation of an Escherichia coli mutant strain lacking SOD activity and by DNA sequence analysis of a cloned fragment. Upstream of sod, separated by a 5-bp intergenic region, lies the open reading frame orfk which potentially codes for a protein of 209 amino acid residues. The amino acid sequence for this presumptive product had a similarity coefficient of 55.5% to a subunit of the alkyl hydroperoxide reductase (encoded by the ahpC gene) from Salmonella typhimurium.
从严格厌氧的嗜热自养甲烷杆菌马堡菌株中分离出一个编码超氧化物歧化酶（SOD）的基因（sod）。通过对缺乏SOD活性的大肠杆菌突变株进行功能互补以及对克隆片段进行DNA序列分析，证实了其身份。在sod上游，由一个5碱基对的基因间隔区隔开，存在一个开放阅读框orfk，它可能编码一个由209个氨基酸残基组成的蛋白质。该推测产物的氨基酸序列与鼠伤寒沙门氏菌的烷基过氧化氢还原酶（由ahpC基因编码）的一个亚基的相似系数为55.5%。
