Sbardellati A, Scarselli E, Tomei L, Kekulé A S, Traboni C
Istituto di Ricerche di Biologia Molecolare P. Angeletti, 00040 Pomezia, Rome, Italy.
J Virol. 1999 Dec;73(12):10546-50. doi: 10.1128/JVI.73.12.10546-10550.1999.
GB virus B (GBV-B) is a virus of the family Flaviviridae that infects small primates (Saguinus sp. [tamarins]) and shows similarities to hepatitis C virus (HCV) in genome organization, protein function, tissue tropism, and pathogenicity. This suggests the possibility of using tamarins infected by GBV-B or GBV-B/HCV chimeric viruses as a surrogate animal model of HCV infection. To achieve the construction of such chimeric viruses, it is essential to produce a complete and infectious GBV-B genomic RNA. We have identified a novel sequence at the 3' end of the GBV-B genome and show that it can be arranged in a secondary structure resembling that of the 3' end of the HCV genome, which is known to be essential for infectivity.
GB病毒B(GBV-B)是黄病毒科的一种病毒,可感染小型灵长类动物(绢毛猴属[绢毛猴]),在基因组结构、蛋白质功能、组织嗜性和致病性方面与丙型肝炎病毒(HCV)相似。这表明使用感染GBV-B或GBV-B/HCV嵌合病毒的绢毛猴作为HCV感染替代动物模型的可能性。为了构建此类嵌合病毒,产生完整且具有感染性的GBV-B基因组RNA至关重要。我们在GBV-B基因组的3'端鉴定出一个新序列,并表明它可以排列成类似于HCV基因组3'端的二级结构,已知该结构对感染性至关重要。