Tarlton J F, Bailey A J, Crawford E, Jones D, Moore K, Harding K D
Collagen Research Group, Division of Molecular and Cellular Biology, University of Bristol, United Kingdom.
Wound Repair Regen. 1999 Sep-Oct;7(5):347-55. doi: 10.1046/j.1524-475x.1999.00347.x.
A 25 patient study was conducted into the relationship between markers of collagen metabolism in venous ulcer exudates and healing status, and their prognostic value in predicting healing performance. Wounds were sampled on at least 5 occasions over 12 months, the frequencies of which were determined by the need for clinic attendance. Specimens were taken from several sites on each ulcer using sterile preweighed filters. Wound margins were traced and sites recorded for each collection. Sample sites were evaluated for severity as improving, static, or deteriorating according to subsequent wound progression. Specimens were analyzed for levels of proenzyme and active forms of matrix metalloproteinases 2 and 9, neutrophil elastase, and type I collagen C propeptide. There was an overall trend of greater expression of all markers with increasing severity of wound site, this being highly significant for pro-matrix metalloproteinase-9 (p = 0.006). For samples collected simultaneously from improving and deteriorating regions of the same wound, paired data analysis showed statistically significant differences for pro-matrix metalloproteinase-9 (p < 0.001), neutrophil elastase (p < 0.005) and activated matrix metalloproteinase-9 (p < 0.05). Taken overall, these data show the potential of markers of collagen biochemistry as predictors of repair in venous ulcers; in particular pro-matrix metalloproteinase-9 and neutrophil elastase were found to be accurate prognostic indicators of subsequent healing.
一项针对25名患者的研究探讨了静脉溃疡渗出物中胶原蛋白代谢标志物与愈合状态之间的关系，以及它们在预测愈合情况方面的预后价值。在12个月内至少对伤口进行5次采样，采样频率根据临床就诊需求确定。使用无菌预称重滤器从每个溃疡的多个部位采集标本。每次采集时都要描绘伤口边缘并记录部位。根据后续伤口进展情况，对采样部位的严重程度进行评估，分为改善、静止或恶化。对标本分析基质金属蛋白酶2和9的酶原和活性形式、中性粒细胞弹性蛋白酶以及I型胶原C端前肽的水平。随着伤口部位严重程度的增加，所有标志物的表达总体上有升高趋势，这在基质金属蛋白酶原-9方面非常显著（p = 0.006）。对于从同一伤口的改善和恶化区域同时采集的样本，配对数据分析显示基质金属蛋白酶原-9（p < 0.001）、中性粒细胞弹性蛋白酶（p < 0.005）和活化基质金属蛋白酶-9（p < 0.05）存在统计学显著差异。总体而言，这些数据表明胶原蛋白生物化学标志物具有作为静脉溃疡修复预测指标的潜力；特别是基质金属蛋白酶原-9和中性粒细胞弹性蛋白酶被发现是后续愈合的准确预后指标。
