Department of Orthopaedics Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, No.109, Xueyuan West Road, Lucheng District, Wenzhou, 325000, Zhejiang, People's Republic of China.
J Orthop Surg Res. 2020 Nov 11;15(1):526. doi: 10.1186/s13018-020-02065-y.
The aim of the study was to evaluate the change of subchondral bone collagen and trabecular bone in the weight-bearing area of femoral head from patients with osteoarthritis (OA) or osteonecrosis of femoral head (ONFH), and discuss the effect of collagen degradation on OA and ONFH.
Femoral heads from patients with femoral neck fracture (FNF) were collected as control group. All collected samples were divided into OA group (N = 10), ONFH group (N = 10), and FNF group (N = 10). Differences of subchondral bone collagen were compared through scanning electron microscope (SEM) observation, immunohistochemistry staining, and Masson's trichrome staining. Alteration of subchondral bone was displayed through hematoxylin and eosin (H&E) staining and gross morphology.
SEM results showed that collagen fibers in OA and ONFH group appeared to be thinner, rougher, sparser, and more wizened. Immunohistochemistry and Masson's trichrome staining results demonstrated that the content of collagen fibers in the OA and ONFH group was obviously less than the FNF group. H&E staining results showed that trabecular bone in OA and ONFH group appeared to be thinner and ruptured. Gross morphology results showed that the degeneration and destruction of cartilage and subchondral bone in OA and ONFH group were severer than FNF group. The characteristics mentioned above in ONFH group were more apparent than OA group.
This study revealed that degradation of collagen fibers from subchondral bone in the weight-bearing area of femoral head was associated with OA and ONFH, which may help to find new therapeutic strategies of the diseases.
本研究旨在评估骨关节炎（OA）或股骨头坏死（ONFH）患者股骨头承重区软骨下骨胶原和小梁骨的变化，并探讨胶原降解对 OA 和 ONFH 的影响。
收集股骨颈骨折（FNF）患者的股骨头作为对照组。所有采集的样本分为 OA 组（N=10）、ONFH 组（N=10）和 FNF 组（N=10）。通过扫描电镜（SEM）观察、免疫组织化学染色和 Masson 三色染色比较软骨下骨胶原的差异。通过苏木精和伊红（H&E）染色和大体形态显示软骨下骨的改变。
SEM 结果显示，OA 和 ONFH 组的胶原纤维显得更细、更粗糙、更稀疏、更干瘪。免疫组织化学和 Masson 三色染色结果表明，OA 和 ONFH 组的胶原纤维含量明显少于 FNF 组。H&E 染色结果显示，OA 和 ONFH 组的小梁骨显得更细且断裂。大体形态结果显示，OA 和 ONFH 组的软骨和软骨下骨退变和破坏比 FNF 组更严重。ONFH 组的上述特征比 OA 组更为明显。
本研究表明，股骨头承重区软骨下骨胶原纤维的降解与 OA 和 ONFH 有关，这可能有助于找到治疗这些疾病的新策略。
