Mitamura K, Sugaya N, Shimizu H, Nirasawa M, Takahashi K, Hirai Y, Takeuchi Y
Department of Pediatrics, Nippon Kokan Hospital.
Kansenshogaku Zasshi. 1999 Oct;73(10):1069-73. doi: 10.11150/kansenshogakuzasshi1970.73.1069.
The optical immunoassay test (FLU OIA, BioStar, USA) for rapid detection of influenza A and B viral antigens was compared with viral isolation in cell culture. A total of 103 respiratory specimens were tested on 75 pediatric patients with acute respiratory illnesses. Influenza viruses were recovered in 40 specimens (type A: 5, Type B: 35). FLU OIA demonstrated 80.0% sensitivity and 68.8% specificity for nasopharyngeal aspirates and 36.7% sensitivity and 83.9% specificity for throat swabs. We also tested FLU OIA, retrospectively, using 78 supernatant samples from pediatric patients with influenza A virus infection frozen after cell culture. FLU OIA demonstrated 91.4% sensitivity and 92.3% specificity for nasopharyngeal aspirates and 50.0% sensitivity and 91.7% specificity for throat swabs diluted in viral transport media. Nasopharyngeal aspirates showed higher sensitivity than throat swabs for detection of influenza virus by FLU OIA. We believe this rapid test kit is useful for the detection of influenza A and B viruses.
将用于快速检测甲型和乙型流感病毒抗原的光学免疫分析测试（FLU OIA，美国BioStar公司）与细胞培养中的病毒分离法进行了比较。对75例患有急性呼吸道疾病的儿科患者的103份呼吸道标本进行了检测。在40份标本中分离出流感病毒（甲型：5份，乙型：35份）。对于鼻咽抽吸物，FLU OIA的灵敏度为80.0%，特异性为68.8%；对于咽拭子，灵敏度为36.7%，特异性为83.9%。我们还回顾性地使用了78份来自甲型流感病毒感染儿科患者的细胞培养后冷冻的上清液样本对FLU OIA进行检测。对于在病毒运输培养基中稀释的鼻咽抽吸物，FLU OIA的灵敏度为91.4%，特异性为92.3%；对于咽拭子，灵敏度为50.0%，特异性为91.7%。通过FLU OIA检测流感病毒时，鼻咽抽吸物的灵敏度高于咽拭子。我们认为这种快速检测试剂盒对于甲型和乙型流感病毒的检测是有用的。
