IJkel Wilfred F J, van Strien Elisabeth A, Heldens Jacobus G M, Broer René, Zuidema Douwe, Goldbach Rob W, Vlak Just M
Laboratory of Virology, Wageningen University and Research Centre, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
J Gen Virol. 1999 Dec;80 ( Pt 12):3289-3304. doi: 10.1099/0022-1317-80-12-3289.
The nucleotide sequence of the DNA genome of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV), a group II NPV, was determined and analysed. The genome contains 135611 bp and has a G+C content of 44 mol%. Computer-assisted analysis revealed 139 ORFs of 150 nucleotides or larger; 103 have homologues in Autographa californica MNPV (AcMNPV) and a further 16 have homologues in other baculoviruses. Twenty ORFs are unique to SeMNPV. Major differences in SeMNPV gene content and arrangement were found compared with the group I NPVs AcMNPV, Bombyx mori (Bm) NPV and Orgyia pseudotsugata (Op) MNPV and the group II NPV Lymantria dispar (Ld) MNPV. Eighty-five ORFs were conserved among all five baculoviruses and are considered as candidate core baculovirus genes. Two putative p26 and odv-e66 homologues were identified in SeMNPV, each of which appeared to have been acquired independently and not by gene duplication. The SeMNPV genome lacks homologues of the major budded virus glycoprotein gene gp64, the immediate-early transactivator ie-2 and bro (baculovirus repeat ORF) genes that are found in AcMNPV, BmNPV, OpMNPV and LdMNPV. Gene parity analysis of baculovirus genomes suggests that SeMNPV and LdMNPV have a recent common ancestor and that they are more distantly related to the group I baculoviruses AcMNPV, BmNPV and OpMNPV. The orientation of the SeMNPV genome is reversed compared with the genomes of AcMNPV, BmNPV, OpMNPV and LdMNPV. However, the gene order in the 'central' part of baculovirus genomes is highly conserved and appears to be a key feature in the alignment of baculovirus genomes.
对Ⅱ类核型多角体病毒——甜菜夜蛾多粒包埋核型多角体病毒（SeMNPV）的DNA基因组核苷酸序列进行了测定和分析。该基因组含有135611 bp，G+C含量为44 mol%。计算机辅助分析显示有139个开放阅读框（ORF）长度在150个核苷酸及以上；其中103个在苜蓿银纹夜蛾核型多角体病毒（AcMNPV）中有同源物，另外16个在其他杆状病毒中有同源物。20个ORF是SeMNPV所特有的。与Ⅰ类核型多角体病毒AcMNPV、家蚕（Bm）核型多角体病毒、黄杉毒蛾（Op）核型多角体病毒以及Ⅱ类核型多角体病毒舞毒蛾（Ld）核型多角体病毒相比，发现SeMNPV的基因组成和排列存在重大差异。85个ORF在所有5种杆状病毒中保守，被视为候选杆状病毒核心基因。在SeMNPV中鉴定出两个假定的p26和odv-e66同源物，它们似乎都是独立获得的，而非通过基因复制。SeMNPV基因组缺乏在AcMNPV、BmNPV、OpMNPV和LdMNPV中发现的主要出芽病毒糖蛋白基因gp64、立即早期反式激活因子ie-2和bro（杆状病毒重复ORF）基因的同源物。杆状病毒基因组的基因同源性分析表明，SeMNPV和LdMNPV有一个较近的共同祖先，且它们与Ⅰ类杆状病毒AcMNPV、BmNPV和OpMNPV的亲缘关系较远。与AcMNPV、BmNPV、OpMNPV和LdMNPV的基因组相比，SeMNPV基因组的方向是相反的。然而，杆状病毒基因组“中央”部分的基因顺序高度保守，似乎是杆状病毒基因组比对的一个关键特征。
