Darkes M J, Davies S M, Bradshaw J P
Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh, UK.
FEBS Lett. 1999 Nov 19;461(3):178-82. doi: 10.1016/s0014-5793(99)01454-4.
The structural effects of the fusion peptide of feline leukemia virus (FeLV) on the lipid polymorphism of N-methylated dioleoylphosphatidylethanolamine were studied using a temperature ramp with sequential X-ray diffraction. This peptide, the hydrophobic amino-terminus of p15E, has been proven to be fusogenic and to promote the formation of highly curved, intermediate structures on the lamellar liquid-crystal to inverse hexagonal phase transition pathway. The FeLV peptide produces marked effects on the thermotropic mesomorphic behaviour of MeDOPE, a phospholipid with an intermediate spontaneous radius of curvature. The peptide is shown to reduce the lamellar repeat distance of the membrane prior to the onset of an inverted cubic phase. This suggests that membrane thinning may play a role in peptide-induced membrane fusion and strengthens the link between the fusion pathway and inverted cubic phase formation. The results of this study are interpreted in relation to models of the membrane fusion mechanism.
利用温度梯度结合连续X射线衍射，研究了猫白血病病毒（FeLV）融合肽对N-甲基二油酰磷脂酰乙醇胺脂质多态性的结构影响。该肽是p15E的疏水氨基末端，已被证明具有融合性，并能在层状液晶到反六角相转变途径上促进高度弯曲的中间结构的形成。FeLV肽对MeDOPE（一种具有中等自发曲率半径的磷脂）的热致介晶行为产生显著影响。结果表明，该肽在反立方相出现之前会减小膜的层间距。这表明膜变薄可能在肽诱导的膜融合中起作用，并加强了融合途径与反立方相形成之间的联系。本研究结果结合膜融合机制模型进行了解释。
