myc家族原癌基因以及相关基因max和mad在滑膜组织中的表达

Expression of the myc-family proto-oncogenes and related genes max and mad in synovial tissue.

作者信息

Roivainen A, Pirilä L, Yli-Jama T, Laaksonen H, Toivanen P

机构信息

Turku Immunology Centre, Department of Medical Microbiology, Turku University, Finland.

出版信息

Scand J Rheumatol. 1999;28(5):314-8. doi: 10.1080/03009749950155517.

DOI:10.1080/03009749950155517

PMID:10568429

Abstract

OBJECTIVE

To examine the expression of myc proto-oncogenes; c-myc, L-myc, and N-myc, and their related genes max and mad, in the arthritic synovium.

METHODS

Using reverse transcription-polymerase chain reaction (RT-PCR), Northern and Southern hybridizations, the expression of these genes in the synovial tissue from rheumatoid arthritis (RA) and osteoarthritis (OA) was analyzed. Synovial specimens from cadavers without any joint disease and peripheral blood mononuclear cells (PBMC) from healthy individuals served as controls.

RESULTS

As a novel finding, synovial cells were observed to express L-myc, N-myc as well as their related genes max and mad, in addition to the previously described presence of c-myc proto-oncogene in synovium. c-myc, L-myc, N-myc, and mad were expressed in all patient samples studied, including the controls. Instead, max was detected in only 10/12 of RA patients, in 11/13 of OA patients, and in all controls (4/4 cadavers, 5/5 blood donors). Six patients with RA revealed positive signals for max only after hybridization. The same was also true of two patients with OA and of one healthy individual donating blood.

CONCLUSIONS

The L-myc, N-myc, max, and mad genes are expressed in synovial cells, in addition to c-myc proto-oncogene. However, expression of these genes is not disease-specific, since they were equally expressed in synovial samples from patients with RA or OA as well as from cadavers representing controls without any joint disease.

摘要

目的

检测myc原癌基因；c-myc、L-myc和N-myc及其相关基因max和mad在关节炎滑膜中的表达。

方法

采用逆转录聚合酶链反应（RT-PCR）、Northern和Southern杂交技术，分析这些基因在类风湿关节炎（RA）和骨关节炎（OA）滑膜组织中的表达。取自无任何关节疾病尸体的滑膜标本以及健康个体的外周血单个核细胞（PBMC）作为对照。

结果

作为一项新发现，除先前已描述的滑膜中存在c-myc原癌基因外，还观察到滑膜细胞表达L-myc、N-myc及其相关基因max和mad。c-myc、L-myc、N-myc和mad在所有研究的患者样本中均有表达，包括对照组。相反，仅在12例RA患者中的10例、13例OA患者中的11例以及所有对照组（4例尸体、5例献血者）中检测到max。6例RA患者仅在杂交后显示max阳性信号。2例OA患者和1例献血健康个体也出现同样情况。